采收期对龙眼果实品质和耐贮性的影响

以福建省主栽龙眼品种油潭本果实为材料，研究采收期(从8月19日到9月12日每隔4 d采果一次)对龙眼果实品质和耐贮性的影响。结果表明：随着采收期的延迟和果实成熟度的提高(8月19日到9月8日采收)，龙眼果皮颜色由青褐色逐渐变成黄褐色，果实由近长圆球形逐渐变成扁圆球形，果实质量和大小逐渐增加，果皮逐渐变薄而果肉逐渐增厚，果肉可食率、营养品质和食用品质提高。但过熟的龙眼(9月12日采收)果皮变成灰褐色，果实品质下降。可用果皮颜色，果实质量和大小，果皮和果肉厚度，果肉可食率、可溶性固形物、总糖、维生素C含量和固     

基于HJ-CCD数据和随机森林算法的小麦叶面积指数反演

为给小麦长势的遥感监测提供技术支持,该文运用随机森林回归(RF,random forest)算法建立小麦叶面积指数(LAI)遥感反演模型。首先基于2010-2013年江苏地区小麦环境减灾卫星HJ-CCD的影像数据,提取拔节、孕穗和开花3个生育期的卫星植被指数,进而根据各生育期植被指数和相应实测LAI数据,利用RF算法构建各期小麦LAI反演模型,并以人工神经网络(ANN,artificial neural network)模型为参比模型进行预测精度的比较。结果表明:RF算法模型在3个生育期的预测结果均好于同期的ANN模型。拔节、孕穗和开花3个生育期RF模型预测值与地面实测值的R2分别为0.79,0.67和0.59,对应的RMSE分别为0.57,0.90和0.78;ANN模型的R2分别为0.67,0.31和0.30,对应的RMSE分别为0.82,1.94和1.43。该研究结果为提高大田尺度下的小麦LAI遥感预测精度提供了技术和方法。     

俄罗斯小麦籽粒硬度及puroindoline基因等位变异的分布

小麦籽粒硬度是重要的品质性状之一,对小麦加工品质具有重要影响.为了合理引进种质资源,培育高品质小麦品种,本研究利用小麦硬度指数测定仪JYDB100×40对引进俄罗斯小麦品种的籽粒硬度进行检测,利用STS标记和变温PCR对控制籽粒硬度基因puroindoline的主要等位变异进行分析.结果显示:208个俄罗斯引进小麦品种硬度指数范围为45.6 ～79.2,硬质麦品种为190个(91.35％),混合麦品种为18个(8.65％).Pina基因有Pina-D1a、Pina-D1b和杂合Pina-D1a/ Pina-D1b共3种类型,所占比例分别为87.02％、10.10％和2.88％.Pinb基因有Pinb-D1a、Pina-D1b、Pinb-D1c和双位点突变Pinb-D1bc 4种类型,所占比例分别为37.02％、55.77％、3.85％和3.37％.统计结果显示:携带Pina-D1b+Pinb-D1a基因小麦籽粒硬度指数显著大于Pina-D1a+Pinb-D1b,Pina-D1a+Pinb-D1a和Pina-D1a+Pinb-D1bc.其他puroindoline等位变异之间没有显著差异.俄罗斯硬质麦种质资源丰富,是改善我国小麦籽粒硬度和品质的重要遗传材料.     

Bioassay-directed Fractionation of Organic Extracts of Marine Surface Sediments from the North and Baltic Sea - Part II: Results of the biotest battery and development of a biotest index (8 pp)

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Part I: Determination and identification of organic pollutants Part II: Results of the biotest battery and development of a biotest index

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Preamble. This series of two papers presents the results of an interdisciplinary research project (ISIS) dealing with bioassay-directed fractionation of marine sediment extracts. Part I presents the extraction and fractionation procedure as well as the results of chemical analysis, including non-target analysis of sediments. Part II describes the results of the biotest battery in relation to chemicals possibly causing parts of the observed effects. A biotest index is used to compare the toxicities of the samples.

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AUTHORS / AFFILIATIONS Ninja Reineke (3), Werner Wosniok (4), Dirk Danischewski (1), Heinrich Hühnerfuss (3), Angelika Kinder (5), Arne Sierts-Herrmann (5), Norbert Theobald (2), Hans-Heinrich Vahl (6), Michael Vobach (1), Johannes Westendorf (6) and Hans Steinhart (5).

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(1) Federal Research Centre for Fisheries, Institute for Fishery Ecology, Palmaille 9, 22767 Hamburg, Germany (2) Federal Maritime and Hydrographic Agency, Bernhard-Nochtstr. 78, 20359 Hamburg, Germany (3) University of Hamburg, Institute for Organic Chemistry, Martin-Luther-King-Platz 6, 20146 Hamburg, Germany (4) University of Bremen, Institute of Statistics, Bibliothekstr. 1, 28334 Bremen, Germany (5) University of Hamburg, Institute for Food Chemistry, Grindelallee 117, 20146 Hamburg, Germany (6) University of Hamburg, University Hospital Hamburg-Eppendorf, Department for Toxicology, Vogt-Kölln-Str. 30, 22527 Hamburg, Germany (7) Eurofins Wiertz-Eggert-Jörissen, Stenzelring 14b, 21107 Hamburg, Germany

Goal, Scope and Background

The ecological relevance of contaminants in mixtures is difficult to assess, because of possible interactions and due to lacking toxicity data for many substances present in environmental samples. Marine sediment extracts, which contain a mixture of environmental contaminants in low concentrations, were the object of this study. The extracts were investigated with a set of different biotests in order to identify the compound or the substance class responsible for the toxicity. For this goal, a combination of biotests, biotest-directed fractionation and chemical analysis has been applied. Further on, a strategy for the development of a biotest index to describe the toxicity of the fractions without a prior ranking of the test results is proposed. This article (Part II) focuses on the biological results of the approach.

Methods

The toxicological potential of organic extracts of sediments from the North Sea and the Baltic Sea was analyzed in a bioassay-directed fractionation procedure with a set of biotests: luciferase reporter gene assays on hormone receptor and Ah receptor, arabinose resistance test, fish embryo test (Danio rerio), comet assay, acetylcholinesterase inhibition test, heat-shock protein 70 induction, oxidative stress and luminescence inhibition test (Vibrio fischeri). The test results provided the basis for the calculation of a biotest index by factor analysis to compare the toxicity of the samples and fractions.

Results and Discussion

Results of 11 biotests on different fractionation levels of the samples were described and discussed with regard to the occurrence of contaminants and their toxic potentials. Polychlorinated biphenyls, polycyclic aromatic hydrocarbons, quinones, brominated indoles and brominated phenols were in the focus of interest. A biotest index was constructed to compare the toxic responses in the samples and to group the biotest results.

Conclusion

The procedure presented in this study is well suited for bioassay-directed fractionation of marine sediment extracts. However, in relatively low contaminated samples, high enrichment factors and sufficient fractionation is necessary to allow identification of low concentrations of contaminants which is required to link effects and possible causes. In the present case, the relation between substances and effects was difficult to uncover due to relatively low concentrations of pollutants compared to the biogenic matrix and to the remaining complexity of the fractions. The results, with respect to the brominated phenols and indoles in the samples, highlight the successful use of bioassay directed fractionation in the case of high concentrations and high toxicity.

Recommendation and Outlook

In general, it has been shown that a marine risk assessment requires focusing on the input of diffuse sources and taking into account the fact of mixture toxicity. Effects resulting from biogenic substances will make the assessment of the influence of anthropogenic substances even more difficult.     

追氮和垄膜沟播种植对晋南旱地冬小麦氮素利用的影响

在自然降水条件下,通过2年大田试验研究了追氮、垄膜沟播种植对晋南旱地冬小麦生育期地上部氮素吸收累积,02 m土层氮素残留、损失及氮肥利用率和氮素平衡的影响。结果表明: 追氮和垄膜沟播种植均可显著提高旱地冬小麦地上部氮素累积量和氮肥利用率,苗期和返青至抽穗期是冬小麦氮素吸收累积的两个高峰期,分别占到最大累积量的20%46%和29%57%。追施氮的利用率高于基施氮的利用率; 随施氮量增加,土壤残留无机氮（Nmin）增加,主要集中在060 cm土层,2040 cm土层为硝态氮积累的峰值区,垄膜沟播种植可以减少土壤残留无机氮（Nmin）,但增加了氮的表观损失量; 冬小麦生长季土壤氮输出以表观损失为主,氮肥表观损失率在4087%7629%之间,且主要发生在播前至返青期。本试验条件下,综合氮素吸收累积、土壤残留、氮肥利用率及氮素平衡等因素,旱地冬小麦应采取氮肥后移顶凌追施和垄膜沟播相配套的种植方式。     

千岛湖水质变化特征与趋势分析

对千岛湖水质综合污染指数分析表明,近10年来千岛湖多数监测项目和各断面污染指数都呈现显著下降的趋势,全湖综合水质趋好,表明千岛湖总体水质的变化正处于相对低的风险状态之中。各断面水污染综合指数的对比分析显示,代表千岛湖主要来水水质的街口、航头岛和百亩畈3个断面分列前三,河流污染物的输入是千岛湖水质下降的主要风险来源,其中街口指标显著高于其他断面,是污染输入强度最大的来源。各监测项目污染指数的对比分析表明总N是影响近期千岛湖水质的首要因子。趋势分析表明,除街口和西园码头外其他各断面负荷比均呈现出不同程度的下降趋势。各监测项目综合污染指数分担率的分析表明,总N、溶解氧和高锰酸钾指数排在前列,3项分担率之和占全湖的71%,其中总N接近49%,显著高于其他项目。趋势分析中总N分担率出现显著下降趋势,其他项目也都表现出不同程度下降趋势,大肠杆菌的变化趋势尚不明确。     

冀南地区蔬菜保护地土壤养分变化特点

对冀南地区蔬菜保护地土壤养分现状及变化趋势进行了研究，结果表明，保护地耕层土壤有机质、土壤速效N、速效P和速效K比相邻露地栽培分别平均增加146．64％、51．68％、567．17％和110．41％；比1981年土壤分别增加143．23％、97．97％、2311．00％和98.8％。随栽培年限的增加，耕层土壤速效N、速效P和速效K有积累的趋势，土壤养分主要积累在0～20cm土层，随着土层加深土壤养分积累程度降低，其中以日光温室栽培的土壤养分积累高于塑料大棚。     

无公害可降解地膜对玉米生长及土壤环境的影响

试验研究无公害可降解地膜对玉米生长及土壤环境的影响结果表明:降解地膜在玉米生长前期具有保温、保水的显著效果,叶面积显著增大,叶片数差异不明显;液态地膜降解最快且无污染,环保作用突出,可在蔬菜等生育期短的作物上应用;生物降解膜降解较慢,具有较好的环保作用,且比露地显著增产,可在生育期较长的作物上推广应用;生物-光降解膜降解最慢,环保效果不明显,但比露地显著增产,适于玉米等生育期长的作物应用。     

日粮添加木聚糖酶对肉鸡小肠葡萄糖吸收及其转运载体基因表达影响

156羽1日龄AA肉雏鸡随机分为对照组和试验组，对照组饲以小麦基础日粮，试验组在基础日粮中添加1000mg/kg木聚糖酶，试验期为30d。与对照组相比，试验组4周内平均日增重提高4.81％（P〈0．01），料重比下降5．00％（P〈0．05）；肠系膜静脉血清中葡萄糖的含量提高5．56％（P〈0．05）。采用半定量RT-PCR方法测定十二指肠和空肠黏膜SGLT1 （sodium／glucose cotransporter 1）和GLUT2（glucose transporter 2）mRNA的表达，发现木聚糖酶显著增加十二指肠SGLT1 mRNA的表达，增幅为33．30％（P〈0．05），试验组空肠SGLT1 mRNA的表达量比对照组增加8．21％，但差异不显著（P〉0．05）。木聚糖酶对十二指肠和空肠GLUT2 mRNA表达均无显著影响。提示木聚糖酶主要作用在小肠上段，通过上调SGLT1的表达而影响肉鸡小肠葡萄糖吸收。     

丘陵红壤橘园不同地面管理措施的经济生态效益研究

在丘陵红壤橘园上．经过3年6种地面管理措施的经济生态效益研究。结果表明：（1）水土流失，以清耕法作对照，生草法、克无踪、草甘膦、草草克、克克草的地表径流量可显著减少分别为38．8％．42．5％．18，7％，28．7％和37．5％；土壤侵蚀量可显著减少分别为55．5‰，51．7％．39．9％．46．8％和50．0％；流失的氮磷钾养分总量可分别减少60．3％．50．2％，37．0％．41．8％和45．4％。（2）杂草再生率，以生草法做对照，则处理中的清耕法、克无踪、草甘膦、草草克和克克草分别是生草法的55．1%．67．2％．30．3％．36．8％和51．2％。（3）土壤肥力，以土壤OM，N,P2O3,K2O总量增减率看，清耕法为-4．4%,生草法7．1％，克无踪6．9％，草甘膦5．3％．克克草6．2％．草草克6．6％。（4）柑橘的产量和品质，以清耕法作对照，除生草法外，克无踪、草甘膦、草草克、克克草均增产7％～10％；可溶性固形物、总糖量、总酸度、糖酸比以及单果重均达到显著差异水平。克无踪调控杂草效果显著．可作为丘陵红壤丘陵园地水保与培肥兼得的有效地面管理措施。